|
KMID : 0613820230330010073
|
|
Journal of Life Science
2023 Volume.33 No. 1 p.73 ~ p.81
|
|
|
Anti-inflammatory Effects of Houttuynia cordata and Lespedeza cuneata on Lipopolysaccharide-stimulated RAW264.7 Cells
|
|
Kim Jeong-Tae
Chung Chung-Wook
Park Seong-Ik
Lee Man-Hyo
Roh Joong-Hee
Sohn Ho-Yong
Kim Jong-Sik
|
|
|
Abstract
|
|
|
In the present study, we prepared hot water extracts and the subsequent organic solvent fractions
of methanol extracts of Houttuynia cordata (HC) and Lespedeza cuneata (LC), and investigated their
anti-inflammatory effects on lipopolysaccharide-stimulated RAW264.7 cells. Among the treated sam- ples, hexane, chloroform, and ethyl-acetate fractions of HC and LC inhibited nitric oxide (NO) pro- duction in a dose-dependent manner, and decreased inducible nitric oxide synthase (iNOS) protein expression. And, we analyzed the flavonoid contents of the ethyl-acetate fraction of HC and LC, and chose apigenin for the further experiments because apigenin was one of flavonoids commonly found in HC and LC. Apigenin dramatically inhibited NO production in a dose-dependent manner
without affecting cell viability and decreased iNOS and cyclooxygenase-2 (COX-2) expression. In addition, apigenin suppressed the phosphorylation of p38 and Jun N-terminal kinase (JNK) indicating
that apigenin exerts anti-inflammatory activity via the mitogen-activated protein kinase (MAPK) signaling pathway. Subsequently, we conducted RNA-sequencing analysis to detect differentially expressed genes upon apigenin treatment. Among the down-regulated genes, four cytokine genes (interleukin (IL)-1α, IL-1β, IL-6, and colony stimulating factor 2 (CSF2)) were selected for the further analysis, and the reduction of their expression by apigenin was confirmed with quantitative real-time polymerase chain reaction. Overall, our results suggest that Houttuynia cordata and Lespedeza cuneata have the anti-inflammatory effects and apigenin can be the one of key molecules responsible for their anti-inflammatory activities.
|
|
|
KEYWORD
|
|
|
Anti-inflammation, apigenin, Houttuynia cordata, Lespedeza cuneata, RNA-seq
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|